Analysing small insect glands with UV-LDI MS: high-resolution spatial analysis reveals the chemical composition and use of the osmeterium secretion in Themira superba (Sepsidae: Diptera).

摘要: For many insect species, pheromones are important communication tools, but chemical analysis and experimental study can be technically challenging because they require the detection handling of complex chemicals in small quantities. One drawback traditional mass spectrometry methods such as gas chromatography is that whole-body extractions from one to several hundred individuals required, with consequence intra- interindividual differences cannot detected. Here, we used recently introduced UV-LDI MS (ultraviolet laser desorption/ionization spectrometry) profile ‘osmeterium’ sepsid fly Themira superba located on edge hind tibia males. Based analyses individual legs, established gland produced a secretion consisted oxygenated hydrocarbons putative isoprenoids. The was first detected 24 h after eclosion, its transfer wings females during mating demonstrated using MS. We then tested whether had an anti-aphrodisiac function, virgin did not affect success or copulation duration. Throughout study, proved invaluable, it allowed tracking natural quantities specific body parts flies.