Seasonal and Growth-Dependent Dynamics of Bacterial Community in Radish Sprouts

摘要: Bacterial counts of 170 radish sprout (Raphanus sativus) retailed in Japan between June 2012 and February 2013 was examined. Aerobic plate coliform exhibited seasonal variations. No Shiga toxin-producing Escherichia coli or Salmonella spp. were detected from them. 16S rRNA pyrosequencing analysis 16 representative samples one farm (farm D) revealed a predominance Pseudomonas throughout seasons, summer an increase Enterobacteriaceae including decreases Oxalobacteraceae (primarily Duganella Janthinobacterium spp.) Flavobacteriaceae (Flavobacterium compared with winter samples. Quantitative-PCR 40 the D confirmed dynamics E. Flavobacterium Radish seeds increased proportion after sprouting, pre-sprouted seeds, suggesting that might be sourced their seeds. Our data thus provides atmospheric control at postsprouting stage hygienic practical point to secure microbial safety. Practical Applications Fresh sprouts are common vehicles foodborne pathogens have been responsible for much numbers outbreaks worldwide. Through use indicator bacterial combination metagenomic approaches, we growth-dependent variation community structure sprouts. Information relief provided manufacturers/consumers could help safety associated fresh