Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis.
作者： Abhaypratap Vishwakarma , Ahan Dalal , Sarada Devi Tetali , Pulugurtha Bharadwaja Kirti , Kollipara Padmasree
关键词:
摘要: This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression AtAOX1a S. cerevisiae. After exposure stress, pYES2AtAOX1a better survival decrease reactive oxygen species (ROS) when compared with empty vector (pYES2). Furthermore, sustained growth by regulating GPX2 and/or TSA2, cellular NAD+/NADH ratio. Thus, enhances its respiratory tolerance which, turn, maintains redox homeostasis protects from damage.
sci-hub.se 参考文章(81)
Daisuke Saisho, Eiji Nambara, Satoshi Naito, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono, Characterization of the gene family for alternative oxidase from Arabidopsis thaliana Plant Molecular Biology. ,vol. 35, pp. 585- 596 ,(1997) , 10.1023/A:1005818507743
Nobuko MINAGAWA, Akio YOSHIMOTO, The induction of cyanide-resistant respiration in Hansenula anomala. Journal of Biochemistry. ,vol. 101, pp. 1141- 1146 ,(1987) , 10.1093/OXFORDJOURNALS.JBCHEM.A121978
Simon Brown, Raewyn Tuffery, Induction of Alternative Oxidase Activity in Candida albicans by Oxidising Conditions World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering. ,vol. 4, pp. 45- 49 ,(2010)
Abhaypratap Vishwakarma, Sarada Devi Tetali, Jennifer Selinski, Renate Scheibe, Kollipara Padmasree, Importance of the alternative oxidase (AOX) pathway in regulating cellular redox and ROS homeostasis to optimize photosynthesis during restriction of the cytochrome oxidase pathway in Arabidopsis thaliana. Annals of Botany. ,vol. 116, pp. 555- 569 ,(2015) , 10.1093/AOB/MCV122
Julio F. Turrens, Superoxide Production by the Mitochondrial Respiratory Chain Bioscience Reports. ,vol. 17, pp. 3- 8 ,(1997) , 10.1023/A:1027374931887
Abhaypratap Vishwakarma, Leena Bashyam, Balasubramanian Senthilkumaran, Renate Scheibe, Kollipara Padmasree, Physiological role of AOX1a in photosynthesis and maintenance of cellular redox homeostasis under high light in Arabidopsis thaliana. Plant Physiology and Biochemistry. ,vol. 81, pp. 44- 53 ,(2014) , 10.1016/J.PLAPHY.2014.01.019
Enrique Herrero, Joaquim Ros, Gemma Bellí, Elisa Cabiscol, Redox control and oxidative stress in yeast cells. Biochimica et Biophysica Acta. ,vol. 1780, pp. 1217- 1235 ,(2008) , 10.1016/J.BBAGEN.2007.12.004
A. G. Rogov, R. A. Zvyagilskaya, Physiological role of alternative oxidase (from yeasts to plants) Biochemistry (Moscow). ,vol. 80, pp. 400- 407 ,(2015) , 10.1134/S0006297915040021
Birgit Arnholdt-Schmitt, José H. Costa, Dirce Fernandes de Melo, AOX--a functional marker for efficient cell reprogramming under stress? Trends in Plant Science. ,vol. 11, pp. 281- 287 ,(2006) , 10.1016/J.TPLANTS.2006.05.001
T. Magnani, F.M. Soriani, V.P. Martins, A.M. Nascimento, V.G. Tudella, C. Curti, S.A. Uyemura, Cloning and functional expression of the mitochondrial alternative oxidase of Aspergillus fumigatus and its induction by oxidative stress. Fems Microbiology Letters. ,vol. 271, pp. 230- 238 ,(2007) , 10.1111/J.1574-6968.2007.00716.X