Community‐Level Analysis: Key Genes of CO2‐Reductive Acetogenesis

摘要: CO2‐reductive acetogenic bacteria are ubiquitous in anaerobic habitats and physiologically phylogenetically diverse. The latter characteristics have rendered their diversity natural environments, distributions, ecological function(s) difficult to assess. Recently introduced polymerase chain reaction (PCR) primers for specific amplification of the structural gene encoding formyltetrahydrofolate synthetase (FTHFS, EC 6.3.4.3), a key enzyme acetyl‐CoA pathway acetogenesis, facilitated studies acetogen ecology. These amplify an approximately 1100‐bp segment FTHFS gene. sequences been recovered from authentic acetogens, sulfate reducing bacteria, variety other nonacetogenic bacteria. Phylogenetic analyses segregated these into distinct clusters, only one which contained known acetogens. This chapter describes PCR primers, defines conditions successful sequences, details phylogenetic analysis sequences. Information on types that how they interpreted is also included.