Molecular imprint-based stationary phases for capillary electrochromatography

摘要: The combination of capillary electrochromatography, which provides a high degree separation efficiency and short times, with molecular imprinting, means for preparing stationary phases predetermined selectivity, is discussed in this review. Polymerisation around templating ligand, using monomers that are selected their ability to participate non-covalent interactions, constitute the key step imprinting. Imprints possessing permanent memory imprint species formed, enabling resultant polymer selectively rebind molecule from mixture closely related compounds. Levels binding affinity selectivity typical antibodies achievable best cases. Several studies have focused on imprinted polymers being used as chiral liquid chromatography (LC) where enantioselectivity given by enantiomeric form ligand. use LC columns, however, hampered poor chromatographic performance, evidenced peak broadening tailing. Being more efficient technique, electrochromatography greatly improves performance polymer-based separations. most successful approach utilises columns filled monolithic, superporous obtained an situ photo-initiated polymerisation process. This technique enables imprint-based systems be operational within 3 h start preparation. Enantiomer separations baseline resolution been carried out less than 2 min. Other approaches (CEC) electrophoresis (CE) involve immobilisation particles inside capillaries polyacrylamide gel, mobile phase additive prepared dispersion