Polymerase chain reaction for detection of Leptospira spp. in clinical samples.

摘要: A sensitive assay for Leptospira spp., the causative agent of leptospirosis, was developed on basis polymerase chain reaction (PCR). 331-bp sequence from interrogans serovar canicola rrs (16S) gene amplified, and PCR products were analyzed by DNA-DNA hybridization using a 289-bp fragment internal to amplified DNA. Specific also obtained with DNA closely related nonpathogenic biflexa but not other spirochetes, such as Borrelia burgdorferi, hermsii, Treponema denticola, pallidum, Spirochaeta aurantia, or more distant organisms Escherichia coli, Staphylococcus aureus, Mycobacterium tuberculosis, Proteus mirabilis. The able detect few 10 bacteria. detected in urine experimentally infected mice. In addition, test found be suitable diagnosing leptospirosis humans. Cerebrospinal fluid patients positive, whereas samples control uninfected negative. Images