A large number of mature and functional dendritic cells can be efficiently generated from umbilical cord blood–derived mononuclear cells by a simple two‐step culture method
作者： Sreekumar Balan , Vaijayanti P. Kale , Lalita S. Limaye
DOI: 10.1111/J.1537-2995.2010.02706.X
关键词:
摘要: BACKGROUND: Advances in the past two decades dendritic cell (DC) biology paved way to exploit them as a promising tool cancer immunotherapy. The prerequisite for DC vaccine preparations is large-scale vitro generations of homogeneous, mature, and functional DCs. Frequent improvements are being made existing production protocols achieve this goal. In our previous study we reported generation DCs from umbilical cord blood (UCB) CD34+ cells. Here report that method can be used efficient UCB mononuclear cells (MNCs) thus hematopoietic stem isolation step not essential. STUDY DESIGN AND METHODS: MNCs or isolated same (CB) samples were characterized morphology, phenotype, assays including antigen uptake, chemotaxis, mixed leukocyte reaction. Similarly generated fresh frozen CB units compared. RESULTS: morphologic, phenotypic, characterization various sets show they comparable nature irrespective starting population used. CONCLUSION: We conclude essential eliminated. More importantly, with equal efficiency units. Our culture will useful exploiting potential an additional source allogeneic clinical settings.
sci-hub.se 参考文章(45)
Shin-ichiro Fujii, Koji Fujimoto, Motomi Osato, Kazuo Matsui, Kiyoshi Takatsuki, Makoto Kawakita, Induction of antitumor cytotoxic activity using CD34+ cord blood cell-derived and irradiated tumor cell-primed dendritic cells. International Journal of Hematology. ,vol. 68, pp. 169- 182 ,(1998) , 10.1016/S0925-5710(98)00069-3
John W. Fabre, The allogeneic response and tumor immunity Nature Medicine. ,vol. 7, pp. 649- 652 ,(2001) , 10.1038/89008
Jean-François Arrighi, Conrad Hauser, Bernard Chapuis, Rudolf H. Zubler, Vincent Kindler, Long-term culture of human CD34(+) progenitors with FLT3-ligand, thrombopoietin, and stem cell factor induces extensive amplification of a CD34(-)CD14(-) and a CD34(-)CD14(+) dendritic cell precursor. Blood. ,vol. 93, pp. 2244- 2252 ,(1999) , 10.1182/BLOOD.V93.7.2244
Roberta Mortarini, Andrea Anichini, Giorgio Parmiani, Alessandro M. Gianni, Alessandra Molla, Filiberto Belli, Massimo Di Nicola, Salvatore Siena, Marco Bregni, Autologous Dendritic Cells Derived from CD34+ Progenitors and from Monocytes Are Not Functionally Equivalent Antigen-presenting Cells in the Induction of Melan-A/Mart-127–35-specific CTLs from Peripheral Blood Lymphocytes of Melanoma Patients with Low Frequency of CTL Precursors Cancer Research. ,vol. 57, pp. 5534- 5541 ,(1997)
Tanja D. de Gruijl, Rik J. Scheper, Chris J. L. M. Meijer, Gert Jan Schuurhuis, Hetty J. Bontkes, Erik Hooijberg, Expansion of dendritic cell precursors from human CD34(+) progenitor cells isolated from healthy donor blood; growth factor combination determines proliferation rate and functional outcome. Journal of Leukocyte Biology. ,vol. 72, pp. 321- 329 ,(2002) , 10.1189/JLB.72.2.321
A FRANZKE, The role of G-CSF in adaptive immunity Cytokine & Growth Factor Reviews. ,vol. 17, pp. 235- 244 ,(2006) , 10.1016/J.CYTOGFR.2006.05.002
Frances Santiago‐Schwarz, Deborah A. Rappa, Karen Laky, Steven E. Carsons, Stem cell factor augments tumor necrosis factor-granulocyte-macrophage colony-stimulating factor-mediated dendritic cell hematopoiesis. Stem Cells. ,vol. 13, pp. 186- 197 ,(1995) , 10.1002/STEM.5530130210
Jun Zhou, Desheng Weng, Fangjian Zhou, Ke Pan, Haifeng Song, Qijing Wang, Huan Wang, Hui Wang, Yongqiang Li, Lixi Huang, Huakun Zhang, Wei Huang, Jianchuan Xia, Patient-derived renal cell carcinoma cells fused with allogeneic dendritic cells elicit anti-tumor activity: in vitro results and clinical responses Cancer Immunology, Immunotherapy. ,vol. 58, pp. 1587- 1597 ,(2009) , 10.1007/S00262-009-0668-9
Andreia R Neves, Luis Felipe C Ensina, Luciene B Anselmo, Katia RM Leite, Antonio C Buzaid, Luiz H Câmara-Lopes, José Alexandre M Barbuto, None, Dendritic cells derived from metastatic cancer patients vaccinated with allogeneic dendritic cell–autologous tumor cell hybrids express more CD86 and induce higher levels of interferon-gamma in mixed lymphocyte reactions Cancer Immunology, Immunotherapy. ,vol. 54, pp. 61- 66 ,(2005) , 10.1007/S00262-004-0550-8
Uwe Trefzer, Guido Weingart, Yingwen Chen, Gunda Herberth, Karin Adrian, Helmut Winter, Heike Audring, Yajun Guo, Wolfram Sterry, Peter Walden, Hybrid cell vaccination for cancer immune therapy: first clinical trial with metastatic melanoma. International Journal of Cancer. ,vol. 85, pp. 618- 626 ,(2000) , 10.1002/(SICI)1097-0215(20000301)85:5<618::AID-IJC4>3.0.CO;2-Z