Chlamydomonas reinhardtii: A Model System for the Genetic Analysis of Flagellar Structure and Motility

摘要: Publisher Summary The chapter discusses the characteristics of Chlamydomonas reinhardtii that make it uniquely useful for genetic analysis flagellar function and structure. presents a review how recent analyses various mutants have contributed to knowledge properties interactions individual proteins in structure flagellum. unicellular green alga C. possesses co mbination makes particularly favorable organism biochemical studies. It is haploid cell, which can be grown vegetatively high densities on defined media, either liquid cultures or as lawns agar plates. Its mitotic division synchronized by use alternating light dark periods, has wide temperature growth range 15–34°C. nuclear system 18 linkage groups been identified. cells undergo classical meiosis, methods production isolation meiotic products unordered tetrads are well established. In Chlamydomonas, single pair motile flagella approximately 10 μm length found at anterior pole cell. typical eukaryotic addition this transient reversal swimming direction, also display other behavioral responses thatare mediated changes activity. exhibit both positive negative phototaxis. kinetics regeneration characterized. describes combined with structural dissecting enormous complexity axoneme.