High-Resolution Microcoil 1H-NMR for Mass-Limited, Nanoliter-Volume Samples

摘要: High-resolution, proton nuclear magnetic resonance (NMR) spectra of 5-nanoliter samples have been obtained with much higher mass sensitivity [signal-to-noise ratio (S/N) per micromole] than traditional methods. Arginine and sucrose show a mean enhancement 130 compared to 278-microliter run in 5-millimeter tube conventional, commercial probe. This can reduce data acquisition time by factor >16,000 or the needed sample about 130. A linewidth 0.6 hertz was achieved on 300-megahertz spectrometer matching susceptibility medium that surrounds detection cell copper coil. For sucrose, limit (defined at S/N = 3) 19 nanograms (56 picomoles) for 1-minute acquisition. technique should prove useful mass-limited use as detector capillary separations.