An HSV-1 Vector Containing the Rat Tyrosine Hydroxylase Promoter Enhances Both Long-Term and Cell Type-Specific Expression in the Midbrain

摘要: A defective herpes simplex virus type one (HSV-1) vector that contains a 6.8-kb fragment of the rat tyrosine hydroxylase promoter (pTHlac-7kb) was examined for its capability to target catecholaminergic cell type-specific expression in CNS. Cell assessed by comparison with control (pHSVlac) uses HSV-1 immediate early 4/5 support multiple types. In initial experiments comparing and noncatecholaminergic lines, pTHlac-7kb supported seven- 20-fold increase reporter gene lines. Four days after stereotactic injection into midbrain adult rats, 10-fold targeting beta-galactosidase hydroxylase-expressing neurons substantia nigra pars compacta compared pHSVlac. Expression from stably maintained 6 weeks no significant changes pattern expression. Long-term confirmed RNA DNA analysis. contrast, pHSVlac decreased approximately 30-fold between 4 transfer. Thus, within context this system, enhanced contributed stable, long-term recombinant product neurons. The specific brain areas may be useful studies on roles these physiology behavior.