A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes.

摘要: Amplification of 8p11–12 is a well-known alteration in human breast cancers but the driving oncogene has not been identified. We have developed high-resolution comparative genomic hybridization array covering and analysed 33 primary tumors, 20 ovarian tumors 27 cancer cell lines. Expression analysis genes region was carried out by using real-time quantitative PCR and/or oligo-microarray profiling. In all, 24% (8/33) 5% (1/20) ovary 15% (4/27) lines showed amplification. identified 1 Mb segment common amplification that excludes previously proposed candidate genes. Some amplified did show overexpression, whereas for others, overexpression specifically attributable to The FLJ14299, C8orf2, BRF2 RAB11FIP, map within minimal amplicon, two putative function consistent with an oncogenic role, these four strong correlation between are therefore best driver oncogenes at 8p12.