Preproteins of chloroplast envelope inner membrane contain targeting information for receptor-dependent import into fungal mitochondria.
作者： M Boutry , U Schmitz , K Becker , S Brink , N Pfanner
DOI: 10.1016/S0021-9258(17)34031-0
关键词:
摘要: The amino-terminal transit sequences of two preproteins destined for the chloroplast inner envelope membrane show similarities to mitochondrial presequences in prevalence positive charges and potential formation an amphipathic alpha-helix. We studied if these could be imported into mitochondria found a low, yet significant import isolated plant mitochondria. were previously shown not precursors stromal or thylakoidal proteins. To analyze specificity we used established systems fungal efficiently Saccharomyces cerevisiae Neurospora crassa Their showed characteristics specific protein uptake, including requirement main receptor MOM19 (mitochondrial outer 19 kDa) across membrane, depended on presence sequence. conclude that some contain sufficient information interaction with receptors (at least from sources).
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