Tissue-specific upregulation of HSP72 in mice following short-term administration of alcohol

摘要: Oxidative stress and cellular injury have been implicated in induction of HSP72 by alcohol. We investigated the association between oxidative mouse tissues following short-term administration high doses alcohol caffeine alone or combination. Adult male C57BL/6J mice were gavaged with vehicle, (∼1.7 g/kg/day), (∼44 mg/kg/day), plus once daily for ten consecutive days. Upon completion treatments, collected structural biochemical analyses. Alcohol caused mild to moderate lesions heart, liver, gastrocnemius muscle. Similar changes observed combined. also led decreased glutathione levels all three reduced plasma superoxide dismutase capacity. In contrast, combination only liver muscle had no effect on dismutase. Significant elevations protein mRNA HSF1 noted caffeine. No significant morphology detected any tested alone. These results suggest that a redox mechanism is involved impairment high-dose tissue may not be associated induction. Induction mediated at both transcriptional translational levels.