Adenosine triphosphatase in isolated bacterial cell membranes.

摘要: Among the recognized morphological features of cells, esternal membrane is one most poorly understood in terms both structure and function, especially with regard to its participation transport processes. In recent years, chemical enzymatic composition isolated cell membranes from a few species bacterial organisms have received attention number investigators (1) after demonstration by Weibull (2) that could be prepared osmotic lysis protoplasts. The finding (3) this laboratory protoplasts Streptococcus jaecalis (ATCC ~9790) treatment lysozyme, has now made it possible isolate organism initiate study constitution. Our first efforts were directed enzyme adenosine triphosphatase, because previous studies intact cells protoplasts, presence was proposed order account for changes permeability occur during glycolysis subsequent cessation (3, 4). present paper, evidence will presented S. contain some properties described. addition, procedures preparation described, inasmuch as these structures not been previously organism.