A Comparison of High-Resolution Melting Analysis With Denaturing High-Performance Liquid Chromatography for Mutation Scanning Cystic Fibrosis Transmembrane Conductance Regulator Gene as a Model

作者: Carl T. Wittwer , Carl T. Wittwer , Elaine Lyon , Elaine Lyon , Lan Szu Chou

DOI: 10.1309/BF3M-LJN8-J527-MWQY

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摘要: High-resolution melting analysis (HRMA) was compared with denaturing high-performance liquid chromatography (dHPLC) for mutation scanning of common mutations in the cystic fibrosis transmembrane conductance regulator gene. We amplified (polymerase chain reaction under conditions optimized or dHPLC) 26 previously genotyped samples exons 3, 4, 7, 9, 10, 11, 13, 17b, and

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