作者: Carl T. Wittwer , Carl T. Wittwer , Elaine Lyon , Elaine Lyon , Lan Szu Chou
DOI: 10.1309/BF3M-LJN8-J527-MWQY
关键词:
摘要: High-resolution melting analysis (HRMA) was compared with denaturing high-performance liquid chromatography (dHPLC) for mutation scanning of common mutations in the cystic fibrosis transmembrane conductance regulator gene. We amplified (polymerase chain reaction under conditions optimized or dHPLC) 26 previously genotyped samples exons 3, 4, 7, 9, 10, 11, 13, 17b, and