Harvesting, Isolation, and Functional Assessment of Primary Vagal Ganglia Cells

摘要: Airway sensory nerves play an important defensive role in the lungs, being central mediating protective responses like cough and bronchoconstriction. In some cases, these become excessive, hypersensitive, deleterious. Understanding normal function of airway phenotype changes associated with disease will help developing new therapeutics for treating chronic obstructive pulmonary cough. Guinea pigs, to a lesser extent ferrets, are commonly employed studying reflex because they have response similar humans. While rats mice do not exhibit response, possess that respond same range tussive stimuli as guinea pigs Described this unit protocols harvesting pig, mouse, rat nerve cell bodies assess molecular functional disease, identify targets therapeutic intervention.