New Quantitative, Qualitative, and Confirmatory Media for Rapid Analysis of Food for Clostridium perfringens1

摘要: A selective and differential medium, Shahidi-Ferguson Perfringens agar (SFP agar), a confirmatory lactose-motility (LM were developed for the enumeration identification of Clostridium perfringens in foods. These media provide rapid, specific, direct diagnosis C. perfringens. SFP contains sodium metabisulfite ferric ammonium citrate to demonstrate H(2)S production egg yolk lecithinase by On agar, produces black colonies, 2 3 mm diameter, surrounded zones opaque precipitate. The typical colonies are confirmed on LM agar. Enumeration completed within 48 hr. All ingredients stable heat storage conditions. also two antibiotics, kanamycin polymyxin B, which inhibitory many bacteria commonly occurring comparative study noninhibitory showed that did not inhibit any 16 strains tested. Recovery added foods averaged 90.6% as compared with 69.8% sulfite polymyxin-sulfadiazine (SPS) (BBL) 60.2% SPS (Difco). much larger consistently black. Of 464 food samples tested, was found 27 5 (Difco), recovery ratio considerably higher is more specific presumptive medium conjunction should save considerable time, effort, materials toward final species.