The effect of specific growth rate and death rate on monoclonal antibody production in hybridoma chemostat cultures
作者： Theodor I. Linardos , Nicolas Kalogerakis , Leo A. Behie , Louis R. Lamontagne
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摘要: Experimental data collected from bench-scale chemostat cultures of mouse-mouse hybridoma cells have been used for the development a kinetic model monoclonal antibody production. The specific productivity was found to be proportional death rate, indicating stimulating effect stress on ability produce antibody. rate an exponential function average cell age in culture. Furthermore, Generalized Maintenance Energy (GME) is proposed, take account effects culture nutrient uptake rates. proposed relationships agreed very well with other literature.
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