Solvent Extraction and Identification of Active Anticariogenic Metabolites in Piper cubeba L. through ¹H-NMR-Based Metabolomics Approach.
作者： Raja Raja Mazlan , Yaya Rukayadi , M. Maulidiani , Intan Ismail
DOI: 10.3390/MOLECULES23071730
关键词:
摘要: The aim of this study was to determine the effects different solvents for extraction, liquid–liquid partition, and concentrations extracts fractions Piper cubeba L. on anticariogenic; antibacterial anti-inflammatory activity against oral bacteria. Furthermore, 1H-Nuclear Magnetic Resonance (NMR) coupled with multivariate data analysis (MVDA) applied discriminate between examine metabolites that correlate bioactivities. All tested bacteria were susceptible fractions. Different partition have partially influenced activity. MTT assay showed P. not toxic RAW 264.7 cells at selected concentrations. Anti-inflammatory evaluated by nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated a reduction NO treated fractions, compared those without treatment. Twelve putative been identified, which are (1) cubebin, (2) yatein, (3) hinokinin, (4) dihydrocubebin, (5) dihydroclusin, (6) cubebinin, (7) magnosalin, (8) p-cymene, (9) piperidine, (10) cubebol, (11) d-germacrene (12) ledol. extraction caused separation principal component (PCA) models. partial least squares (PLS) models higher anticariogenic related more polar solvents, despite some active also present non-polar solvents. Hence, exhibited potential be developed as agent.
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