Molecular Facets of Pluripotency

摘要: Early mammalian embryogenesis is characterized by a gradual restriction in the developmental potential of cells that constitute embryo. The inner cells, called cell mass (ICM), generate all lineages embryo proper, but they cannot contribute to trophoblast, and thus are widely considered be pluripotent. These vitro substitutes for embryos search genetic switches molecular mechanisms required ensure pluripotency. They provide suitable model system study cellular commitment differentiation, have one major disadvantage: embryonal carcinoma (EC) tumor consequently, typically aneuploid. In addition EC embryonic stem (ES) third type pluripotent cell, germ (EG) has been isolated from mouse. ES self-renewal dependence on cytokine supply can attributed several factors: leukemia inhibitory factor (LIF) may influence rate proliferation or cycle progression, act phenotype activating signaling cascade operates up- down-regulation genes exclusively expressed “pluripotent” differentiated respectively.