Method for maintaining high-quality focus during high-throughput, microscopic digital montage imaging

摘要: Today's technology allows montage images of large areas to be captured, stored and displayed at the resolution limit microscope optics. The invention reduces overall time capture a slide by reducing overhead associated with refocusing optics each tile location. Using macroscopic image region scanned, representative focus positions are selected based on predefined set characteristics. Prior scanning, these placed under best-focus position determined. A surface is fit resulting three-dimensional data. parameters that define this feed into scanning control component allow high-quality focused acquired throughout process, eliminating required stop, refocus, acquire processing steps used in traditional imaging systems.