Mild chemically cleavable linker system

摘要: A linker system is provided where a small molecule reactive group, e.g., an activity based probe which binds to certain enzymes at the active site, linked through aryl diazo affinity such as biotin. The group may comprise number of functionalities known react with specific target be studied. This enables exposed analytes, proteins and bind specifically them form complex having allowing immobilization bound analyte on column or other support, e.g. streptavidin. Then, cleaved without causing removal dissociation from analyte. under mild reducing conditions, dithionite. synthesized along solid support.