Synthesis and testing of chromogenic phenoxazinone substrates for β-alanyl aminopeptidase

摘要: Novel 7-N-(β-alanyl)aminophenoxazin-3-one salts 27a–d have been synthesized and tested as chromogenic substrates for β-alanyl aminopeptidase, which is present in Pseudomonas aeruginosa, the most common respiratory pathogen patients with cystic fibrosis. The biological results show that 7-N-(β-alanyl)amino-1-pentylphenoxazin-3-one trifluoroacetate salt27a a substrate this bacterium, low degree of diffusion nutrient media growing bacterial cultures bright red colour, making it easily distinguishable from agar background.