Hexose transport in growing petunia pollen tubes and characterization of a pollen-specific, putative monosaccharide transporter.
作者： Bauke Ylstra , Dolores Garrido , Jacqueline Busscher , Arjen J. van Tunen
DOI: 10.1104/PP.118.1.297
关键词:
摘要: We investigated the molecular and physiological processes of sugar uptake metabolism during pollen tube growth plant fertilization. In vitro germination assays showed that petunia (Petunia hybrida) can germinate grow not only in medium containing sucrose (Suc) as a carbon source, but also monosaccharides glucose (Glc) or fructose (Fru). Furthermore, high-performance liquid chromatography analysis demonstrated rapid complete conversion Suc into equimolar amounts Glc Fru when was cultured 2% Suc. This indicates presence wall-bound invertase activity sugars form by growing tube. A cDNA designated pmt1 (petunia monosaccharide transporter 1), which is highly homologous to transporters, isolated from petunia. Pmt1 belongs small gene family expressed specifically male gametophyte, any other vegetative floral tissues. activated after first mitosis, high levels mRNA accumulate mature germinating pollen. model describing transport style, Fru, active presented.
plantphysiol.org
vumc.nl参考文章(31)
N. Sauer, K. Friedländer, U. Gräml-Wicke, Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana. The EMBO Journal. ,vol. 9, pp. 3045- 3050 ,(1990) , 10.1002/J.1460-2075.1990.TB07500.X
Robert G. Stanley, H. F. Linskens, Carbohydrates and Cell Walls Springer Berlin Heidelberg. pp. 129- 144 ,(1974) , 10.1007/978-3-642-65905-8_9
Norbert Sauer, Widmar Tanner, The hexose carrier from Chlorella FEBS Letters. ,vol. 259, pp. 43- 46 ,(1989) , 10.1016/0014-5793(89)81489-9
T. Caspari, R. Stadler, N. Sauer, W. Tanner, Structure/function relationship of the Chlorella glucose/H+ symporter. Journal of Biological Chemistry. ,vol. 269, pp. 3498- 3502 ,(1994) , 10.1016/S0021-9258(17)41890-4
H. Schlupmann, A. Bacic, S. M. Read, Uridine Diphosphate Glucose Metabolism and Callose Synthesis in Cultured Pollen Tubes of Nicotiana alata Link et Otto. Plant Physiology. ,vol. 105, pp. 659- 670 ,(1994) , 10.1104/PP.105.2.659
JAN DERKSEN, TWAN RUTTEN, TON VAN AMSTEL, ANNA DE WIN, FIONA DORIS, MARTIN STEER, Regulation of Pollen Tube Growth Acta Botanica Neerlandica. ,vol. 44, pp. 93- 119 ,(1995) , 10.1111/J.1438-8677.1995.TB00773.X
Jack Kyte, Russell F. Doolittle, A simple method for displaying the hydropathic character of a protein Journal of Molecular Biology. ,vol. 157, pp. 105- 132 ,(1982) , 10.1016/0022-2836(82)90515-0
R. Stadler, K. Wolf, C. Hilgarth, W. Tanner, N. Sauer, Subcellular localization of the inducible Chlorella HUP1 monosaccharide-H+ symporter and cloning of a Co-induced galactose-H+ symporter. Plant Physiology. ,vol. 107, pp. 33- 41 ,(1995) , 10.1104/PP.107.1.33
D. O'Driscoll, C. Hann, S. M. Read, M. W. Steer, Endocytotic uptake of fluorescent dextrans by pollen tubes grown in vitro Protoplasma. ,vol. 175, pp. 126- 130 ,(1993) , 10.1007/BF01385010
R.E. Koes, C.E. Spelt, H.J. Reif, P.J.M. van den Elzen, E. Veltkamp, J.N.M. Mol, Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family Nucleic Acids Research. ,vol. 14, pp. 5229- 5239 ,(1986) , 10.1093/NAR/14.13.5229