Expression of unusual immunophenotype combinations in acute myelogenous leukemia.

摘要: Immunophenotypes for 272 patients with acute myelogenous leukemia (AML) were analyzed using a panel of 22 antibodies. Numerical evidence unusual coexpressions (present in normal marrow at < or = 0.1%) surface markers on 10% the blast cells was found 85% all cases. Asynchronous expression myeloid differentiation antigens occurred 70% Unusual coexpression T-lymphoid, B- lymphoid, natural killer (NK) 38%, 13%, and 21%, respectively, AML Two- three- color analyses confirmed 15 cases, indicated that these percentages are an underestimate, because can be demonstrated cases without numerical overlap. These data indicate is common occurrence AML. Markers 12 13 similar between presentation relapse, two patients, phenotypes detected first relapse shown second indicating immunophenotypes stable majority patients. Significant correlations t(8;21) cytogenetics CD19 CD15 CD34, t(9;22) t(15;17) CD2 antigens. Multiparameter fluorescence analysis allows detection when counts 5% (classical remission). Analysis 16 remission presence 0.2% to 7.9% lymphocyte + light scatter region, representing 0.03% 1.4% total nucleated cells. Of 4 months follow-up after cells, 6 phenotypic have relapsed, while 9 10 remain remission. The phenotype may reflect residual their increase predict relapse.