Reviewing data reduction methods for ektacytometry

摘要: Ektacytometry quantifies erythrocyte deformability by measuring the elongation of suspended red blood cells subjected to a range shear stresses. Raw stress-elongation index plots are difficult interpret and thus data reduction methods characterizing relationship using few parameters without loss information good reproducibility essential, especially for clinician. Two such curve fitting formulas, used widely in literature this purpose, reviewed herein. The Lineweaver-Burke method overestimates maximal if stresses below 1 Pa applied. A modified version formula estimates deformation more accurately but gives little weight at low Neither is accurate negative indices present (artifact phenomenon when measurement performed from high stresses). Streekstra-Bronkhorst provides efficient though theoretical background incorrect. have expressive meaning; however, both minimal deformations slightly underestimated. Moreover, biased according measured