Changes in the concentrations of auxins and polyamines during rooting of in-vitro-propagated walnut shoots.
作者： M.-C. Heloir , C. Kevers , J.-F. Hausman , T. Gaspar
DOI: 10.1093/TREEPHYS/16.5.515
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摘要: Abstract Rooting was induced in in-vitro-propagated walnut (Juglans regia L.) shoots by subculturing the on rooting medium containing agar and 3 mg l(-1) indole-3-butyric acid (IBA) for 7 days darkness. Changes concentrations of endogenous free indole-3-acetic (IAA), indole-3-acetylaspartic (IAAsp) polyamines were determined during culture root-inducing medium. In extracts whole shoots, concentration IAA showed a transient peak at 60 h (around 48 from basal shoot portions) then remained relatively low remainder 7-day period. The IAAsp peaked about same time as IAA, whereas portions earlier, around 12 h. increased soon after transferred to stable when rooted vermiculite/gelrite mixture (without auxin) grown light.
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