[1] Cloning firefly luciferase
作者： Jeffrey R. de Wet , Keith V. Wood , Donald R. Helinski , Marlene DeLuca
DOI: 10.1016/0076-6879(86)33050-7
关键词:
摘要: Publisher Summary This chapter discusses determination of cloning firefly luciferase. Firefly luciferase catalyzes the ATP-dependent oxidative decarboxylation luciferin (LH 2 ) resulting in production light. The most well characterized is isolated from common North American Photinus pyralis . Isolating cDNA clones will allow nucleotide sequence this gene which amino acid enzyme can be deduced. When genes other species fireflies are cloned, comparison their sequences help pinpoint regions that essential to its function and increase our understanding bioluminescent reactions. expression an active form Escherichia coli , would provide unlimited source enzyme. Cloned could very useful for monitoring expression. simple sensitive assay may vivo detection obviating need destroy cells being monitored. As a single system, it likely particularly eukaryotic systems as indicator promoter activity plant, animal, lower eukaryote using both vitro tests.
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