Distribution of cells expressing human renin-promoter activity in the brain of a transgenic mouse

摘要: Abstract Renin plays a critical role in fluid and electrolyte homeostasis by cleaving angiotensinogen to produce Ang peptides. Whilst it has been demonstrated that renin mRNA is expressed the brain, distribution of cells responsible for this expression remains uncertain. We have used transgenic mouse approach an attempt address question. A mouse, which 12.2 kb fragment human promoter was drive Cre-recombinase, crossed with ROSA26- lac Z reporter strain. Cre-recombinase mediated excision floxed stop cassette resulted protein, β-galactosidase. This study describes β-galactosidase brain mouse. In all cases where examined protein co-localized neuronal marker NeuN. An extensive observed numerous labeled somatosensory, insular, piriform retrosplenial cortices. The motor cortex devoid cells. Several other regions were including parts amygdala, periaqueductal gray, lateral parabrachial nucleus deep cerebellar nuclei. Overall shows little overlap those are known express receptors renin–angiotensin system adult brain. approach, demonstrates activated at any time throughout development, yields unique putative renin-expressing neurons. Our observations suggest may broader actions indicate potential interaction (pro)renin receptor or production ligand non-AT 1 /AT 2 receptors.