Use of electric cell–substrate impedance sensing to assess in vitro cytotoxicity

摘要: Abstract In vitro assessment of cytotoxicity based on electrochemical impedance spectroscopy (EIS) needs more quantitative methods to analyze the alteration cell morphology and motility, hence potential risk human health. Here, we applied electric cell–substrate sensing (ECIS) evaluate dose-dependent responses umbilical vein endothelial cells exposed cytochalasin B. To detect subtle changes in morphology, frequency-dependent data monolayer were measured analyzed with a theoretical cell–electrode model. alternation micromotion response B challenge, time-series fluctuations cell-covered electrodes monitored values power spectrum, variance, variance increments calculated verify difference. While relationship was generally observed from overall resistance monolayer, analysis distinguished levels as low 0.1 μМ. Our results show that causes decrease junctional between cells, an increase membrane capacitance, reduction micromotion.