Method for inducing sporulation of pure cultures of the myxomycete Physarum polycephalum.

摘要: Daniel, John W. (University of Wisconsin, Madison) and Harold P. Rusch. Method for inducing sporulation pure cultures the myxomycete Physarum polycephalum. J. Bacteriol. 83:234–240. 1962.—Techniques polycephalum are described. Small plasmodia grown in agitated culture were harvested, allowed to fuse into large (1.3 g wet weight each), and, after incubation dark on a salts medium, exposed light 2 hr. The ensuing formation sporangia containing spores was complete 12 16 obligatory conditions are: (i) an optimal growth age occurring just prior maximal organism at time when nutrients medium exhausted; (ii) 4 days only inorganic niacin or tryptophan; (iii) subsequent illumination with wavelengths between 350 500 mμ.