3-Aminobenzamide--a PARP inhibitor enhances the sensitivity of peripheral blood micronucleus and comet assays in mice.

摘要: AbstractContext: DNA repair is an essential outcome of damage, which may compromise the end point various in vitro and vivo test systems genotoxicity evaluation. poly(ADP-ribose) polymerase (PARP) enzymes have role repair. Here, we investigated effect 3-AB, a PARP inhibitor on sensitivity comet PBMN assays.Objective: This study aimed to enhance peripheral blood micronucleus (PBMN) assays using 3-aminobenzamide (3-AB), well-characterized inhibitor.Materials methods: Cyclophosphamide (CP, 50 mg/kg), 5-flourouracil (5-FU, 25 mg/kg), zidovudine (AZT, 400 mg/kg) furosemide (FUR, 60 mg/kg) were selected as genotoxins. 3-AB was given every 8 h with first dose 2 h before genotoxin treatment. For assay, small amount taken from tail tip each animal smears prepared. The assay performed PBL, bone marrow liver.Results: In well a...