TCR repertoire profiling of tumors, adjacent normal tissues, and peripheral blood predicts survival in nasopharyngeal carcinoma

摘要: The T-cell immune responses in nasopharyngeal carcinoma patients have been extensively investigated recently for designing adoptive immunotherapy or checkpoint blockade therapy. However, the distribution characteristics of T cells associated with NPC pathogenesis are largely unknown. We performed deep sequencing TCR repertoire profiling on matched tumor/adjacent normal tissue from 15 and peripheral blood 39 patients, other diseases, 33 healthy controls. found that a lower diversity tumors than paired tissues low similarity between was poor prognosis NPC. A more diverse identified relative to controls; this related significant decrease proportion high-frequency clones Higher worse prognosis. Due peculiarity Vβ gene usage patterns genes were selected distinguish controls by least absolute shrinkage selection operator analysis. 11 clonotypes shared samples different defined as "NPC-associated" might value immunotherapy. In conclusion, we here report systematic overall tumors, adjacent tissues, patients. data obtained may be relevant future clinical studies setting