Dextran-conjugated tetrathiatriarylmethyl radicals as biocompatible spin probes for EPR spectroscopy and imaging.

作者: Martin Poncelet , Benoit Driesschaert , Oxana Tseytlin , Mark Tseytlin , Timothy D. Eubank

DOI: 10.1016/J.BMCL.2019.05.017

关键词:

摘要: Tetrathiatriarylmethyl (TAM) radicals represent soluble paramagnetic probes for biomedical electron resonance (EPR)-based spectroscopy and imaging. There is an increasing demand in the development of multifunctional, biocompatible targeted trityl hampered by difficulties derivatization TAM structure. We proposed a new straightforward synthetic strategy using click chemistry covalent conjugation radical with water-soluble carrier exemplified here dextran. A set dextran-grafted varied degrees Finland loading dextran modification polyethelene glycol has been synthesized. The EPR spectrum optimized macromolecular probe exhibits single narrow line high sensitivity to oxygen advantages over unbound being insensitive interactions albumin. In vivo imaging tissue oxygenation performed breast tumor-bearing mouse demonstrates its utility preclinical oximetric applications.

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