The preparation and characterization of polycaprolactone/graphene oxide biocomposite nanofiber scaffolds and their application for directing cell behaviors

摘要: Biocomposite nanofiber scaffolds of polycaprolactone (PCL) with different graphene oxide (GO) concentrations were prepared using electrospinning technology. The successful incorporation GO nanosheets into PCL polymer nanofibers improved the thermal and mechanical properties because intrinsic developed due to interactions PCL. In order estimate biocompatibility PCL/GO composite scaffolds, mouse marrow mesenchymal stem cells (mMSCs) low-differentiated rat pheochromocytoma (PC12-L) cultured. Initial adhesion spreading mMSCs PC12-L on moderate addition (0.3 0.5 wt%) significantly superior those pure exhibited typical fibroblast- neuron-like morphologies obvious pseudopods mature appearance, respectively. A small amount did not restrain proliferation viability cells, which indicated appreciable cell affinity GO. Moreover, hybridization dramatically enhanced differentiation osteo- neuro-like All these results suggest that could be a promising alternative material as biocompatible for tissue engineering.