Enterotoxigenic Staphylococcus aureus in bulk milk in Norway

作者: H.J. Jørgensen , T. Mørk , H.R. Høgåsen , L.M. Rørvik

DOI: 10.1111/J.1365-2672.2005.02569.X

关键词:

摘要: ABSTRACT H.J. JORGENSEN, T. MORK, H.R. HOGA˚ SEN AND L.M. RORVIK. 2005. Aims: To investigate the presence of enterotoxigenic Staphylococcus aureus in bulk milk and a selection rawmilk products.Methods results: Samples bovine (n ¼ 220) caprine 213) milk, raw products(n 82) were analysed for S. aureus. Isolates tested staphylococcal enterotoxin (SE) production (SEA-SED) by reversed passive latex agglutination SE genes (sea–see, seg–sej) multiplex PCR. Staphylococcusaureus was detected 165 (75%) 205 (96AE2%) samples 31 (37AE8%) product samples. Enterotoxin observed 22AE1% 57AE3% isolates frombovine respectively, while 52AE5% and55AE8% isolates. SEC sec most commonly detected. A greater diversity SEgenes vs isolates.Conclusions: seems highly prevalent Norwegian frequently produceSEs contain genes. Enterotoxigenic also found products.Significance Impact Study: mayconstitute risk with respect to food poisoning from products.Keywords: enterotoxins, products,

参考文章(49)
J. McLAUCHLIN, G. L. NARAYANAN, V. MITHANI, G. O'NEILL, The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction. Journal of Food Protection. ,vol. 63, pp. 479- 488 ,(2000) , 10.4315/0362-028X-63.4.479
Paula I. Menzies, Siti Z. Ramanoon, Mastitis of Sheep and Goats Veterinary Clinics of North America: Food Animal Practice. ,vol. 17, pp. 333- 358 ,(2001) , 10.1016/S0749-0720(15)30032-3
Edmund A. Zottola, Lorraine B. Smith, Growth and Survival of Undesirable Bacteria in Cheese Cheese: chemistry, physics and microbiology. Volume 1. General aspects.. pp. 471- 492 ,(1993) , 10.1007/978-1-4615-2650-6_12
Yves Le Loir, Michel Gautier, Florence Baron, Staphylococcus aureus and food poisoning. Genetics and Molecular Research. ,vol. 2, pp. 63- 76 ,(2003)
Y C Su, A C Wong, Identification and purification of a new staphylococcal enterotoxin, H. Applied and Environmental Microbiology. ,vol. 61, pp. 1438- 1443 ,(1995) , 10.1128/AEM.61.4.1438-1443.1995
J R Roberson, L K Fox, D D Hancock, T E Besser, Evaluation of methods for differentiation of coagulase-positive staphylococci. Journal of Clinical Microbiology. ,vol. 30, pp. 3217- 3219 ,(1992) , 10.1128/JCM.30.12.3217-3219.1992
N P Kokan, M S Bergdoll, Detection of low-enterotoxin-producing Staphylococcus aureus strains. Applied and Environmental Microbiology. ,vol. 53, pp. 2675- 2676 ,(1987) , 10.1128/AEM.53.11.2675-2676.1987
Sophie Jarraud, Grégoire Cozon, François Vandenesch, Michèle Bes, Jerome Etienne, Gerard Lina, Involvement of Enterotoxins G and I in Staphylococcal Toxic Shock Syndrome and Staphylococcal Scarlet Fever Journal of Clinical Microbiology. ,vol. 37, pp. 2446- 2449 ,(1999) , 10.1128/JCM.37.8.2446-2449.1999
TORE TOLLERSRUD, KEVIN KENNY, DOMINIQUE A. CAUGANT, ARVE LUND, Characterisation of isolates of Staphylococcus aureus from acute, chronic and subclinical mastitis in cows in Norway. Apmis. ,vol. 108, pp. 565- 572 ,(2000) , 10.1034/J.1600-0463.2000.D01-98.X
M. Zschöck, D. Botzler, S. Blöcher, J. Sommerhäuser, H.P. Hamann, Detection of genes for enterotoxins (ent) and toxic shock syndrome toxin-1 (tst) in mammary isolates of Staphylococcus aureus by polymerase-chain-reaction International Dairy Journal. ,vol. 10, pp. 569- 574 ,(2000) , 10.1016/S0958-6946(00)00084-4