Genetic data indicate that proteins containing the GGDEF domain possess diguanylate cyclase activity

摘要: A conserved domain, called GGDEF (referring to a central sequence pattern), is detected in many procaryotic proteins, often various combinations with putative sensory-regulatory components. Most sequenced bacterial genomes contain several different proteins. The function of this domain has so far not been experimentally shown. Through genetic complementation using genes from three bacteria encoding proteins domains as the only element common, we present data indicating (a) that responsible for diguanylate cyclase activity these and (b) cellulose synthase Rhizobium leguminosarum bv. trifolii Agrobacterium tumefaciens regulated by cyclic di-GMP Acetobacter xylinum.