Nrf2 protects photoreceptor cells from photo-oxidative stress induced by blue light

摘要: Abstract Oxidative stress plays a key role in age-related macular degeneration and hereditary retinal degenerations. Light damage rodents has been used extensively to model oxidative stress-induced photoreceptor degeneration, photo-oxidative injury from blue light is particularly damaging photoreceptors. The endogenous factors protecting photoreceptors stress, including are continuing be elucidated. In this study, we evaluated the effect of exposure on its relationship Nrf2 using cultured murine (661W) cells. 661W cells were exposed at 2500 lux. Exposure for 6–24 h resulted significant increase intracellular reactive oxygen species (ROS) death time-dependent fashion. Blue activation Nrf2, as indicated by an nuclear translocation Nrf2. This was associated with induction expression well array target genes, antioxidant quantitative reverse transcription PCR (qRT-PCR). order determine functional siRNA-mediated knockdown studies performed. Nrf2-knockdown exacerbation light-induced levels cell death. Taken together, these findings indicate that important protective factor against suggests drugs targeting could considered neuroprotective strategy AMD other conditions.