Prediction of retention times in serially linked open-tubular gas chromatographic columns and optimization of column lengths

摘要: The serial gas chromatographic theory, first advanced and then further developed by Purnell Williams, is used to optimize the analysis of a nine-component phenol mixture use linked capillary columns. capacity factors sample components with system serially coupled in either mode, column A/column B or B/column A, different relative length, are shown agree essentially exactly those predicted theory. window procedure devised Laub calculate overall length columns required achieve base-line resolution. experimental chromatograms correspond precisely prediction. When methodology applied generally, it that requisite data can be determined easily rapidly subsequent calculations carried out via an equally simple computer program. consequent techniques procedures should help development serial-coupled open-tubular chromatography (SCOTCH).