Molecular cloning, mRNA expression and functional characterization of a catalase from Chinese black sleeper (Bostrychus sinensis).
作者： Bin Shen , Ke Wei , Yuehan Ding , Jianshe Zhang
DOI: 10.1016/J.FSI.2020.04.056
关键词:
摘要: Abstract In this study, the catalase gene of Chinese black sleeper Bostrychus sinensis (termed as BsCat) was sequenced and characterized. The BsCat, which encodes 525 amino acids, contains a proximal active site signature domain (64FDRERIPERVVHAKGAG80) heme-ligand (354RLFAYPDTH362). BsCat exhibits high sequence similarity with Cat other species. Phylogenetic tree reconstruction revealed close evolutionary relationship to genes fishes. results Real-time PCR showed that constitutively expressed in most organs B. sinensis, predominant expression detected liver, followed by peripheral blood spleen. Moreover, significantly changed after either poly (I:C) stimulation or Vibrio parahemolyticus infection blood, head kidney, liver enzymatic activity purified recombinant (rBsCat) 2261 ± 96 U/mg. rBsCat optimum at 15 °C pH 7.0. Our suggested is involved antioxidant defense host immune response during pathogen invasion.
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