A new drying method of biological specimens for scanning electron microscopy: the t-butyl alcohol freeze-drying method.

作者: Hitoshi OSATAKE , Takao INOUÉ

DOI: 10.1679/AOHC.51.53

关键词:

摘要: … bell jar of a vacuum evaporator (HUS-4GB, Hitachi Ltd., Japan), … 5C) to be frozen in a refrigerator, it is preferable that the … be easily frozen in a refrigerator without using liquid nitrogen or …

参考文章(12)
Keiichi Tanaka, Akira Mitsushima, A preparation method for observing intracellular structures by scanning electron microscopy Journal of Microscopy. ,vol. 133, pp. 213- 222 ,(1984) , 10.1111/J.1365-2818.1984.TB00487.X
Robert E. Stowell, Effect on Tissue Volume of Various Methods of Fixation, Dehydration, and Embedding Biotechnic & Histochemistry. ,vol. 16, pp. 67- 83 ,(1941) , 10.3109/10520294109106200
A. Boyde, C. Wood, Preparation of animal tissues for surface-scanning electron microscopy. Journal of Microscopy. ,vol. 90, pp. 221- 249 ,(1969) , 10.1111/J.1365-2818.1969.TB00709.X
A. Boyde, E. MacOnnachie, Freon 113 freeze-drying for scanning electron microscopy Scanning. ,vol. 2, pp. 164- 166 ,(1979) , 10.1002/SCA.4950020306
Keiichi Tanaka, Akihiro Iino, Critical point drying method using dry ice. Biotechnic & Histochemistry. ,vol. 49, pp. 203- 206 ,(1974) , 10.3109/10520297409116978
Alan Boyde, Frantisek Franc, Freeze-drying shrinkage of glutaraldehyde fixed liver. Journal of Microscopy. ,vol. 122, pp. 75- 86 ,(1981) , 10.1111/J.1365-2818.1981.TB01244.X
Acetonitrile Vacuum Drying Method Journal of Electron Microscopy. ,vol. 28, pp. 145- 145 ,(1979) , 10.1093/OXFORDJOURNALS.JMICRO.A050163
Junichi TOKUNAGA, Masanobu EDANAGA, Tsuneo FUJITA, Kazuo ADACHI, Freeze cracking of scanning electron microscope specimens. A study of the kidney and spleen. Archives of Histology and Cytology. ,vol. 37, pp. 165- 182 ,(1974) , 10.1679/AOHC1950.37.165