An anchorage nuclear structure for telomeric DNA repeats in HeLa cells.

摘要: Using either a biotinylated peptide nucleic acid (PNA) oligomer or digoxigenin-labeled double-stranded DNA probe, we determined the distribution of telomeric repeats in HeLa cells by in-situ hybridization at ultrastructural level. The was found periphery previously unrecognized roundish nuclear structures, distributed throughout nucleoplasm. levels association with these structures investigated exposure to detergent-containing hypotonic solution which only preserves tightly linked components. stayed associated their anchorage following spreading apart nucleoproteins. Because changes cellular topology are intranuclear development herpes simplex virus type 1 (HSV-1) and adenovirus 5 (Ad5) cells, examined when is pushed toward border. circular complexes were morphologically unmodified; however, as result partition viral two concentric compartments, they migrated towards border, along compressed chromatin. Taken together, our results exemplify unique organization DNA, coiled around central core diameter 120 nm can therefore be clearly distinguished from bulk