The C. elegans peroxidasin PXN-2 is essential for embryonic morphogenesis and inhibits adult axon regeneration

摘要: Peroxidasins form a highly conserved family of extracellular peroxidases unknown cellular function. We identified the C. elegans peroxidasin PXN-2 in screens for mutants defective embryonic morphogenesis. find that is essential specific stages morphogenesis and muscle-epidermal attachment, also required postembryonically basement membrane integrity. The peroxidase catalytic activity necessary these developmental roles. pxn-2 display aberrant ultrastructure matrix, suggesting role consolidation. affects axon guidance choice points developing nervous system but dispensable maintenance process positions. In adults, loss function promotes regrowth axons after injury, providing first evidence matrix can play an inhibitory regeneration. Loss closely related pxn-1 does not cause overt defects. Unexpectedly, mutant phenotypes are suppressed by exacerbated overexpression wild-type pxn-1, indicating PXN-1 have antagonistic functions. These results demonstrate peroxidasins crucial roles development reveal new as inhibitors axonal