Genome-wide analysis of Dof family transcription factors and their responses to abiotic stresses in Chinese cabbage.

摘要: Chinese cabbage is an important leaf vegetable that experienced long-term cultivation and artificial selection. Dof (DNA-binding One Zinc Finger) transcription factors, with a highly conserved domain, are members of major plant-specific factor family play roles in many plant biological processes. The one the most families transcriptional regulators higher plants, involved massive aspects growth, development, response to abiotic stresses. Our study will supply resources for understanding how factors respond stress interaction network these genes tolerance mechanism. In this study, we performed comprehensive analysis cabbage. total, 76 encoding BraDof were identified from cabbage, those divided into nine classes. Fifteen motifs found based on amino acid sequence alignments. Chromosome locations gene duplications also analyzed. Ten duplicate events discovered chromosomes. uneven distribution Brassica chromosomes may cause expansion genes. family, 37 7 orthologous between Arabidopsis Oryza sativa, respectively. networks constructed. Expression profiles selected different classes subjected four stresses (cold, heat, salt drought) further investigated by quantitative real-time PCR obtain better functions regulation mechanisms two varieties, ‘Lubaisanhao’ ‘Qingdao 87-114’. Dof-family analyzed genome Chromosomal showed duplication might result expansion. Response was elucidated varieties. results provide novel insights responses promote construction function Dofs