Genomic characterization of three urinary bladder cancer cell lines: understanding genomic types of urinary bladder cancer
作者： Rosário Pinto-Leite , Isabel Carreira , Joana Melo , Susana Isabel Ferreira , Ilda Ribeiro
DOI: 10.1007/S13277-013-1604-3
关键词:
摘要: Several genomic regions are frequently altered and associated with the type, stage progression of urinary bladder cancer (UBC). We present characterization 5637, T24 HT1376 UBC cell lines by karyotyping, fluorescence in situ hybridization (FISH), array comparative (aCGH) multiplex ligation-dependent probe amplification (MLPA) analysis. Some cytogenetic anomalies were found three lines, such as chromosome 20 aneuploidy loss 9p21. gene loci losses (e.g. CDKN2A) gains HRAS, BCL2L1 PTPN1) coincident across all lines. Although some significant heterogeneity complexity detected between them, their profiles exhibited a similar pattern to UBC. suggest that 5637 represent E2F3/RB1 pathway due 6p22.3, concomitant one copy RB1 mutation remaining copy. The presented 10q deletion involving PTEN region no alteration PIK3CA which, combination inactivation TP53, bears more invasive metastatic properties than 5637. belongs alternative FGFR3/CCND1 presenting mutated HRAS over-represented CCND1. These cover frequent subtypes reliable models can be used, group, preclinical studies.
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