Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats.
作者： Qun Wu , Qinxue Dai , Liuming Jiang , Yichuan Wang , Tao Yang
关键词:
摘要: The present study aimed to investigate the potential role of microRNA‑448 (miR‑448) in isoflurane-induced learning and memory impairment rats. Sprague‑Dawley rats were used for construction isoflurane‑treated models. Morris water maze test was evaluate effects isoflurane on regarding following para-meters: Swimming speed, escape latency time original quadrant. Influences neuron apoptosis miR‑448 expression rat hippocampus tissue analyzed by flow cytometry reverse transcription‑quantitative polymerase chain reaction, respectively. Furthermore, cell apoptosis‑associated proteins investigated cytometry. results demonstrated that treatment induced higher lower spent quadrant compared with control In addition, highly expressed hippocampal Bcl‑x significantly downregulated while caspase‑3 upregulated an inhibitor. Combined current indicate knockdown may have pivotal roles improving via suppressing apoptosis.
spandidos.info参考文章(35)
Sarah Azoubel Lima, Amy E. Pasquinelli, Identification of miRNAs and Their Targets in C. elegans Systems Biology of RNA Binding Proteins. ,vol. 825, pp. 431- 450 ,(2014) , 10.1007/978-1-4939-1221-6_12
Bin Zhu, Jie Ding, Zhi-Gang Wang, Chun Yang, Isoflurane anesthesia aggravates cognitive impairment in streptozotocin-induced diabetic rats. International Journal of Clinical and Experimental Medicine. ,vol. 7, pp. 903- 910 ,(2014)
J. Varghese, N. S. Khandre, A. Sarin, Caspase-3 activation is an early event and initiates apoptotic damage in a human leukemia cell line Apoptosis. ,vol. 8, pp. 363- 370 ,(2003) , 10.1023/A:1024121017841
Chainllie Young, Vesna Jevtovic-Todorovic, Yue-Qin Qin, Tatyana Tenkova, Haihui Wang, Joann Labruyere, John W Olney, Potential of ketamine and midazolam, individually or in combination, to induce apoptotic neurodegeneration in the infant mouse brain. British Journal of Pharmacology. ,vol. 146, pp. 189- 197 ,(2005) , 10.1038/SJ.BJP.0706301
Yujuan Li, Minting Zeng, Weiqiang Chen, Chuiliang Liu, Fei Wang, Xue Han, Zhiyi Zuo, Shuling Peng, Dexmedetomidine Reduces Isoflurane-Induced Neuroapoptosis Partly by Preserving PI3K/Akt Pathway in the Hippocampus of Neonatal Rats PLoS ONE. ,vol. 9, pp. e93639- ,(2014) , 10.1371/JOURNAL.PONE.0093639
Henrik Viberg, Emma Pontén, Per Eriksson, Torsten Gordh, Anders Fredriksson, Neonatal ketamine exposure results in changes in biochemical substrates of neuronal growth and synaptogenesis, and alters adult behavior irreversibly. Toxicology. ,vol. 249, pp. 153- 159 ,(2008) , 10.1016/J.TOX.2008.04.019
Q-Q Li, Z-Q Chen, X-X Cao, J-D Xu, J-W Xu, Y-Y Chen, W-J Wang, Q Chen, F Tang, X-P Liu, Z-D Xu, Involvement of NF-κB/miR-448 regulatory feedback loop in chemotherapy-induced epithelial-mesenchymal transition of breast cancer cells. Cell Death & Differentiation. ,vol. 18, pp. 16- 25 ,(2011) , 10.1038/CDD.2010.103
Changsheng Li, Sufang Liu, Ying Xing, Feng Tao, The Role of Hippocampal Tau Protein Phosphorylation in Isoflurane-Induced Cognitive Dysfunction in Transgenic APP695 Mice Anesthesia & Analgesia. ,vol. 119, pp. 413- 419 ,(2014) , 10.1213/ANE.0000000000000315
M GAROFALO, G CONDORELLI, C CROCE, MicroRNAs in diseases and drug response. Current Opinion in Pharmacology. ,vol. 8, pp. 661- 667 ,(2008) , 10.1016/J.COPH.2008.06.005
Stephen A. Johnson, Chainllie Young, John W. Olney, Isoflurane-induced neuroapoptosis in the developing brain of nonhypoglycemic mice. Journal of Neurosurgical Anesthesiology. ,vol. 20, pp. 21- 28 ,(2008) , 10.1097/ANA.0B013E3181271850