Quantitative Trait Loci for Freezing Tolerance in a Lowland x Upland Switchgrass Population.

摘要: Low-temperature related abiotic stress is an important factor affecting winter survival in lowland switchgrass when grown northern latitudes the United States. A better understanding of genetic architecture freezing tolerance will aid development cultivars with improved survival. The objectives this study were to conduct a assessment, generate map using single nucleotide polymorphism (SNP) markers, and identify QTL (quantitative trait loci) associated × upland population. pseudo-F2 mapping population was generated from initial cross between Ellsworth cultivar Summer. segregating progenies screened for controlled-environment facility. Two clonal replicates each genotype tested at six different treatment temperatures ranging -15 -5°C interval 2°C two time periods. Tiller emergence (days) tiller number recorded following recovery hypothesis that source higher early emergence. Survivorship ranged 89% 5% -15°C average LT50 -9.7°C. Genotype had significant effect on all traits except -15°C. linkage constructed bi-allelic markers exome capture sequencing. final consisted 1618 2626 cM, inter-marker distance 1.8 cM. Six identified, one chromosomes 1K, 5K, 5N, 6K, 6N, 9K, traits: number, days LT50. comparative genomics revealed genes/proteins, such as COR47, DREB2B, zinc finger-CCCH, WRKY, GIGANTEA, HSP70, NRT2, among others reside within 1.5 LOD confidence identified QTL.