Towards Systems Metabolic Engineering of PHA Producers

摘要: Polyhydroxyalkanoates (PHAs) are natural polyesters that accumulate in numerous microorganisms as a carbon- and energy-storage material under the nutrient-limiting condition presence of an excess carbon source. PHAs considered to be one potential alternatives petrochemically derived plastics owing their versatile properties. Over past few decades, extensive detailed biochemical, molecular-biological, metabolic studies related PHA biosynthesis have been carried out. Advances our knowledge led development engineered strains fermentation processes for production with high efficiency. Even though traditional engineering based on rational thinking trial-and-error type approaches allowed improved strains, further enhancement performance is expected through systems engineering, which integrated systems-biological approaches. In this chapter, strategies taken producers briefly reviewed. Then, genomic proteomic performed understand context whole cell metabolism well develop Finally, suggested; these will make it possible produce higher efficiencies tailor-made by systems-level optimization network establishment novel pathways.