Semiquantitative in vitro binding assay of indium-111-labeled monoclonal antibodies to human cancer and normal tissues.

摘要: We have developed a simple in vitro method for the semiquantitative assessment of radiolabeled antibody binding to cancer and normal tissues. Indium-111-labeled F(ab')2 fragments 17-1A 19-9 monoclonal antibodies with well-characterized specificity gastrointestinal demonstrated similar properties between cultured cells membrane fractions homogenates prepared from tumor All 17 colon specimens seven (64%) 11 gastric obtained by surgery showed positive 17-1A. Specific was observed 9 (53%) cancers 4 (36%) cancers. However, some tissues were also /sup 111/In-labeled Relative levels CA antigen expression, determined antibodies, correlated percent immunohistochemical assays. Furthermore, could be cryopreserved without losing antibody-binding activity. These results indicate that this assay can used testing immunoreactivity anti-tumor