Association of single nucleotide polymorphisms within cytokine genes with juvenile idiopathic arthritis in the Czech population.

摘要: OBJECTIVE: To examine the possible association of juvenile idiopathic arthritis (JIA) with polymorphisms within cytokine genes in Czech population. METHODS: In a case-control study, genotypes 130 patients JIA (63 male, 67 female; age at onset 7.6 +/- 4.4 yrs; 43 oligoarticular, 72 polyarticular, 15 systemic form) were compared to 102 healthy unrelated blood donors. Using polymerase chain reaction technique sequence-specific primers from 13th IHWG workshop, we analyzed 19 single nucleotide 12 different [interleukin (IL)-1a, IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12, transforming growth factor (TGF)-beta, interferon (IFN)-g], and related molecules (IL-1R, IL-1RA, IL-4Ra). Genotype frequencies using chi-square analysis, significance level was corrected for number independent tests. RESULTS: Significant positive found G allele IL-4 -1098 T/G polymorphism, which carried by 10% cases 25% controls [odds ratio (OR) 0.32, 95% confidence interval (CI) 0.16-0.67, p = 0.038). Also, nonsignificant increase frequency IL-1beta +3962 C detected (96%) versus (84%) (OR 4.65, CI 1.64-13.2, 0.091). We did not replicate previously associations IL-1a, IL-1RA polymorphisms. CONCLUSION: Our study showed polymorphism. It also underlines genetic contribution IL-1 pathogenesis JIA, as another polymorphism may influence risk disease.